📝 Homogenize the tissue sample using a homogenizer. Weigh 2.00±0.05 g of the homogenized tissue sample into a 10 mL centrifuge tube, add 3 mL of sample extraction solution, and vortex for 2 min to mix thoroughly. Centrifuge at 4000 r/min for 5 min. Transfer 2 mL of the upper organic phase to a 10 mL centrifuge tube and evaporate to dryness under a nitrogen or air stream in a water bath at 50–60°C. Add 250 µL of sample reconstitution solution and vortex for 1 min to obtain the test solution.

Homogenize the tissue sample using a homogenizer. Weigh 2.00±0.05 g of the homogenized tissue sample into a 10 mL centrifuge tube, add 3 mL of sample extraction solution, and vortex for 2 min to mix thoroughly. Centrifuge at 4000 r/min for 5 min. Transfer 2 mL of the upper organic phase to a 10 mL centrifuge tube and blow dry under a nitrogen or air flow in a 50–60°C water bath. Add 250 µL of sample reconstitution solution and vortex for 1 min; this is the test solution.

🧪 Test Procedure

Read the operation manual carefully before experiment. Before use, equilibrate the test card and test sample to room temperature (20～25℃). Remove the reagent bucket and test strip bag from the original packaging. Open and take out the required number of microwell reagents and test cards. Mark them and use immediately. Using a pipette, transfer 100 μL of test sample solution into a microwell. Slowly aspirate and mix thoroughly with the microwell reagent. After incubation at room temperature for 3 min, using a pipette, transfer approximately 100 μL of the mixed solution and dispense vertically into the sample well (S well). Alternatively, using the disposable dropper provided in the kit, aspirate the mixed solution and add 3 drops vertically and slowly into the sample well (S well). Start timing when liquid begins to flow. React for 8 min. Immediately interpret the result according to the diagram. Results read at other times are invalid.