📝 Homogenize the tissue sample using a homogenizer. Weigh 3.00 ± 0.05 g of the homogenized tissue sample into a 15 mL centrifuge tube, add 5 mL of Malachite Green Reagent A, shake up and down, then vortex for 2 minutes until fully mixed, and centrifuge at 4000 rpm for 3 minutes. First, add 100 μL of Malachite Green Reagent B to a 7 mL centrifuge tube, then add 3 mL of the supernatant after centrifugation, and finally add 150 μL of Malachite Green Reagent C, vortex for 30 s to mix, and centrifuge at 4000 rpm for 3 minutes. Add 140 μL of Malachite Green Reagent D to a 2 mL centrifuge tube, carefully aspirate 70 μL of the lower layer solution after centrifugation (from the very bottom of the centrifuge tube, avoiding the organic layer) and add it, then pipette up and down 5–8 times or vortex for 30 s. This is the test solution. Note: Only use a red marker pen for labeling during the experiment. If the pH of the test sample solution is not between 6 and 8, …

Homogenize the tissue sample using a homogenizer. Weigh 3.00±0.05 g of the homogenized tissue sample into a 15 mL centrifuge tube, add 5 mL of Malachite Green Reagent A, shake up and down, then vortex for 2 minutes until fully mixed, and centrifuge at 4000 rpm for 3 minutes. First add 100 µL of Malachite Green Reagent B into a 7 mL centrifuge tube, then add 3 mL of the supernatant after centrifugation, and finally add 150 µL of Malachite Green Reagent C, vortex for 30 s to mix, and centrifuge at 4000 rpm for 3 minutes. Add 140 µL of Malachite Green Reagent D into a 2 mL centrifuge tube, carefully aspirate 70 µL of the lower layer solution after centrifugation (from the very bottom of the centrifuge tube, avoiding the organic layer) and add it, then repeatedly pipette up and down 5–8 times, or vortex for 30 s. This is the test solution. Note: Use only a red marker for labeling during the experiment. If the pH of the test sample solution is not between 6 and 8.

🧪 Test Procedure

Before testing,, Please Firstread the operating instructions carefully. Before testing, please read the operating instructions carefully. Before use, allow the test cassette and the sample solution to reach room temperature. Remove the reagent barrel and test strip bag from the original packaging, open them, and take out the required number of microwell reagents and test cassettes, and mark them. Use them within 60 minutes as soon as possible. Use a micropipette to aspirate 100 µL of the sample solution into the microwell, slowly pipette and mix thoroughly with the microwell reagent. Incubate at room temperature for 3 minutes, then aspirate 100 µL of the mixed solution and vertically add it into the sample well (S well). Start timing when the liquid flows, react for 5 minutes, determine the result according to the schematic diagram or read the detection result using a colloidal gold analyzer. Other time points are considered invalid.