📝 Homogenize the tissue sample using a homogenizer. Weigh 2.50±0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 4 mL of Reagent A and 150 µL of derivatization reagent, and shake the sample up and down to mix until it becomes a paste. Place the centrifuge tube in an 80°C water bath and incubate for 10 min. Remove the centrifuge tube, add 1 mL of Reagent B, cap the tube, mix well, and cool to room temperature (20–25°C). Add 5 mL of extraction solvent, cap the tube, and invert the tube 20–25 times; do not vortex. Centrifuge at 4000r/min for 5 min (if the supernatant after centrifugation is less than 3 mL, shake the sample up and down 3–4 times and re-centrifuge). Transfer 3 mL of the upper layer into a 10 mL centrifuge tube, and evaporate to dryness under a nitrogen or air stream in a 50–60°C water bath. Add 0.8 mL of cleaning agent, vortex for 1 min, then add 500 µL of sample reconstitution solution, and vortex for 5–6 s to mix thoroughly. Centrifuge at 4000 r/min for 5 min; it is strongly recommended to remove the upper organic phase. The remaining clear liquid is the test solution, which should be tested immediately.

Homogenize the tissue sample using a homogenizer. Weigh 2.50 ± 0.05 g of the homogenized sample into a 15 mL centrifuge tube. Add 4 mL of Reagent A and 150 μL of derivatization reagent, then shake the sample up and down to mix until it forms a paste. Place the centrifuge tube in an 80°C water bath and incubate for 10 min. Remove the centrifuge tube, add 1 mL of Reagent B, close the tube cap, mix, and cool to room temperature (20–25°C). Add 5 mL of extraction solvent, close the tube cap, and invert the tube 20–25 times. Do not vortex. Centrifuge at 4000 r/min for 5 min (if the supernatant after centrifugation is less than 3 mL, shake the sample up and down 3–4 times and re-centrifuge). Transfer 3 mL of the upper layer to a 10 mL centrifuge tube and dry under a nitrogen stream or air stream in a 50–60°C water bath. Add 0.8 mL of cleanup agent, vortex for 1 min, then add 500 μL of sample reconstitution solution and vortex for 5–6 s to mix thoroughly. Centrifuge at 4000 r/min for 5 min. It is strongly recommended to remove the upper organic phase. The remaining clear liquid is the test solution, which should be tested immediately.

🧪 Test Procedure

Before testing,, Please Firstread the operating instructions carefully. Before testing, carefully read the instruction manual. Bring the test card and the sample solution to room temperature before use. Remove the test card from its original packaging, mark it, and use it immediately. Using a micropipette, vertically dispense 75 µL of the sample solution into the sample well (S well), or use the disposable pipette provided in the kit to vertically and slowly add 2–3 drops of the sample solution into the sample well (S well). Start timing when the liquid flows, allow the reaction to proceed for 10 min, and immediately determine the result according to the schematic diagram or read the result using a colloidal gold analyzer. Readings at other times are invalid.