📝 Take the defatted tissue sample and homogenize it in a homogenizer. Weigh 2.00 ± 0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 2 mL of NaCl solution, vortex for 2 min, then add 3 mL of Sample Extraction Solution 1 and 500 μL of Sample Extraction Solution 2, vortex for 3 min, and centrifuge at 3000–4000 r/min for 5 min. Transfer 1 mL of the middle organic phase into a 10 mL centrifuge tube and evaporate to dryness under a nitrogen or air stream in a 50–60 °C water bath. Add 300 µL of Sample Reconstitution Solution, vortex for 20 s, and the resulting solution is the test solution. Note: If the volume taken is less than 1 mL, shake to resuspend and centrifuge again.

Take the defatted tissue sample and homogenize it in a homogenizer. Weigh 2.00 ± 0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 2 mL of NaCl solution, vortex for 2 min, then add 3 mL of Sample Extraction Solution 1 and 500 μL of Sample Extraction Solution 2, vortex for 3 min, and centrifuge at 3000–4000 r/min for 5 min. Transfer 1 mL of the middle organic phase to a 10 mL centrifuge tube, and evaporate to dryness under a nitrogen or air stream in a 50–60 °C water bath. Add 300 μL of Sample Reconstitution Solution, vortex for 20 s, and the solution is ready for testing. Note: If the sample volume taken is less than 1 mL, shake to disperse and then centrifuge again.

🧪 Test Procedure

Before the experiment, please read the operating instructions carefully. Before use, allow the test cassette and the sample solution to equilibrate to room temperature. Remove the reagent barrel and test cassette bag from the original packaging, open them, take out the required number of microwell reagents and test cassettes, mark them, and use immediately. Using a micropipette, aspirate 200 µL of the sample solution into a microwell, slowly pipette up and down to mix thoroughly with the microwell reagent. Incubate at room temperature (20~25°C) for 3 min, then aspirate approximately 100 μL of the mixed solution and add it vertically into the sample well (S well). Start timing when the liquid flows. React for 10 min. Interpret the result according to the schematic diagram or read the detection result using a colloidal gold reader. Interpretations made at other times are invalid.