📝 Homogenize the tissue sample using a homogenizer. Weigh 2.50±0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 4 mL of Reagent A and 150 µL of derivatization reagent, and shake the sample up and down to mix until a paste forms. Place the centrifuge tube in an 80°C water bath and incubate for 10 min. Remove the centrifuge tube, add 1 mL of Reagent B, cap the tube, mix, and cool to room temperature (20–25°C). Add 5 mL of extraction solvent, cap the tube, and invert the tube 20–25 times. Do not vortex. Centrifuge at 4000 r/min for 5 min (if the supernatant after centrifugation is less than 3 mL, shake the sample up and down 3–4 times and re-centrifuge). Transfer 3 mL of the upper layer into a 10 mL centrifuge tube and dry under a nitrogen or air stream in a 50–60°C water bath. Add 0.8 mL of cleanup agent, vortex for 1 min, then add 500 µL of sample reconstitution solution, and vortex for 5–6 s to mix thoroughly. Centrifuge at 4000 r/min for 5 min. It is strongly recommended to remove the upper organic phase; the remaining clear liquid is the test solution and should be analyzed immediately.

Homogenize the tissue sample using a homogenizer. Weigh 2.50±0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 4 mL of Reagent A and 150 µL of derivatization reagent, and shake the tube up and down to mix the sample into a paste. Place the centrifuge tube in an 80°C water bath and incubate for 10 min. Remove the centrifuge tube, add 1 mL of Reagent B, close the cap, mix, and cool to room temperature (20–25°C); add 5 mL of extractant, close the cap, and invert the tube 20–25 times. Do not vortex. Centrifuge at 4000 r/min for 5 min (if the supernatant after centrifugation is less than 3 mL, shake the tube up and down 3–4 times and re-centrifuge). Transfer 3 mL of the upper layer to a 10 mL centrifuge tube and dry under a nitrogen or air flow in a 50–60°C water bath. Add 0.8 mL of purifier, vortex for 1 min, then add 500 µL of sample reconstitution solution and vortex for 5–6 s to mix thoroughly. Centrifuge at 4000 r/min for 5 min. It is strongly recommended to remove the upper organic phase. The remaining clear liquid is the test solution and should be tested immediately.

🧪 Test Procedure

Before testing,, Please Firstread the operating instructions carefully. Before testing, please read the operating instructions carefully. Before use, allow the test cassette and the sample solution to equilibrate to room temperature. Remove the test cassette from the original packaging, mark it, and use immediately. Using a micropipette, aspirate 75 µL of the sample solution and add it vertically into the sample well (S well), or use the disposable dropper provided in the kit to aspirate the sample solution and add 2~3 drops vertically and slowly into the sample well (S well). Start timing when the liquid flows. React for 10 min. Immediately interpret the result according to the schematic diagram or read the detection result using a colloidal gold analyzer. Interpretations made at other times are invalid.