Sample Prep

Homogenize the defatted tissue sample in a homogenizer. Weigh 4.00±0.05 g of the homogenized sample into a 15 mL centrifuge tube, add 4 mL of Reagent A, vortex for 2 min, then add 1 mL of Reagent B, vortex for 2 min. Add 5 mL of extraction solvent, close the centrifuge tube cap, vortex to mix for 1 min, centrifuge at 4000 r/min for 5 min. Transfer 3 mL of the upper organic phase into a 10 mL centrifuge tube, then add 50 μL of protectant, vortex for 5–6 s to mix thoroughly, and evaporate to near dryness under a nitrogen stream at 40–50°C. Add 300 μL of sample diluent, vortex for 30 s to obtain the test solution. [Note: 1. The drug is volatile; when approximately 50 μL of liquid remains at the bottom of the centrifuge tube during nitrogen evaporation, remove immediately. The laboratory reference time for nitrogen evaporation is 5 min. 2. If emulsification occurs, break the emulsion and perform a second centrifugation.]

Detection

before the experiment, Before the experiment, carefully read the instructions. Before use, allow the test card and the sample to reach room temperature. Remove the reagent barrel and test strip bag from the original packaging, open them, and take out the required number of microwell reagents and test strips. Label them and use immediately. Using a micropipette, aspirate 100 μL of the sample solution into a microwell. Slowly aspirate and mix thoroughly with the reagent in the microwell. After incubation at room temperature (20–25°C) for 3 min, aspirate 100 μL of the mixed solution into the sample well (S well). Start timing when the liquid flows. React for 8 min. Interpret the result according to the schematic diagram or read the result using a colloidal gold analyzer. Results at other times are invalid.